In vitro antiplasmodial activity of Withania frutescens —Solanaceae
Identifieur interne : 000092 ( France/Analysis ); précédent : 000091; suivant : 000093In vitro antiplasmodial activity of Withania frutescens —Solanaceae
Auteurs : Laila El Bouzidi ; Widad Ben Bakrim ; Valerie Mahiou-Leddet [France] ; Nadine Azas [France] ; Mustapha Larhsini ; Mohamed Markouk ; Evelyne Ollivier [France] ; Khalid BekkoucheSource :
- European Journal of Integrative Medicine [ 18763820 ] ; 2017-09.
English descriptors
- mix :
Abstract
Introduction: Drugs resistant Plasmodium falciparum is a recurring issue that threatens public health. New anti-plasmodial drugs are needed to overcome this problem. The aim of this study was to characterize in vitro an-tiplasmodial activity of Withania frutescens (Solanaceae) against chloroquine-resistant Plasmodium falciparum strain. Methods: The in vitro antiplasmodial activity of leaves and roots extracts from Withania frutescens was performed in 96 well plates and preliminary phytochemical analysis was performed for the active fractions. The toxicity of the plant extract against CHO (Chinese Hamster Ovary) cells was assessed using the tetrazolium salt MTT col-orimetric assay. The selectivity index (SI) was calculated as the ratio between the cytotoxic and antiparasitic activities. Results: The methanol extract of W. frutescens leaves showed a good antiplasmodial activity (CC 50 18.1 μg/ml). Furthermore, ethyl acetate and butanol fractions showed promising in vitro antiplasmodial activity with a se-lectivity index of 3.1 and 2.4, respectively. The roots of W. frutescens were found to be inactive with a CC 50 value > 80 μg/ml. Conclusion: The antiplasmodial activity of W. frutescens may in part be attributed to the presence of polyphenolic and flavonoid compounds. Based on our results, ethyl acetate and butanol leaves fractions could be considered as a promising source for the development of putative antiplasmodial drugs.
Url:
DOI: 10.1016/j.eujim.2017.08.009
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: 000135
- to stream Hal, to step Curation: 000135
- to stream Hal, to step Checkpoint: 000048
- to stream Main, to step Merge: 000C63
- to stream Main, to step Curation: 000C63
- to stream Main, to step Exploration: 000C63
- to stream France, to step Extraction: 000092
Links to Exploration step
Hal:hal-01765799Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">In vitro antiplasmodial activity of Withania frutescens —Solanaceae</title><author><name sortKey="El Bouzidi, Laila" sort="El Bouzidi, Laila" uniqKey="El Bouzidi L" first="Laila" last="El Bouzidi">Laila El Bouzidi</name></author><author><name sortKey="Ben Bakrim, Widad" sort="Ben Bakrim, Widad" uniqKey="Ben Bakrim W" first="Widad" last="Ben Bakrim">Widad Ben Bakrim</name></author><author><name sortKey="Mahiou Leddet, Valerie" sort="Mahiou Leddet, Valerie" uniqKey="Mahiou Leddet V" first="Valerie" last="Mahiou-Leddet">Valerie Mahiou-Leddet</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-188653" status="VALID"> <idno type="RNSR">201220339K</idno> <orgName>Institut méditerranéen de biodiversité et d'écologie marine et continentale</orgName> <orgName type="acronym">IMBE</orgName> <date type="start">2012</date> <desc> <address> <addrLine>Aix Marseille Université, Campus Etoile, Faculté St-Jérôme case 421 Av. . escadrille Normandie-Niemen 13397 MARSEILLE CEDEX 20</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.imbe.fr/</ref> </desc> <listRelation> <relation name="UMR7263" active="#struct-195507" type="direct"></relation> <relation name="UMR7263" active="#struct-198056" type="direct"></relation> <relation name="UMR7263" active="#struct-302715" type="direct"></relation> <relation name="UMR7263" active="#struct-441569" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR7263" active="#struct-195507" type="direct"><org type="institution" xml:id="struct-195507" status="VALID"> <orgName>Avignon Université</orgName> <orgName type="acronym">AU</orgName> <desc> <address> <addrLine>74 rue Louis Pasteur - 84 029 Avignon cedex 1</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-avignon.fr/</ref> </desc> </org></tutelle><tutelle name="UMR7263" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno> <idno type="ISNI">0000 0001 2176 4817</idno> <orgName>Aix Marseille Université</orgName> <orgName type="acronym">AMU</orgName> <date type="start">2012-01-01</date> <desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-amu.fr/</ref> </desc> </org></tutelle><tutelle name="UMR7263" active="#struct-302715" type="direct"><org type="institution" xml:id="struct-302715" status="VALID"> <orgName>Institut de recherche pour le développement [IRD] : UMR237</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR7263" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Azas, Nadine" sort="Azas, Nadine" uniqKey="Azas N" first="Nadine" last="Azas">Nadine Azas</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-220808" status="OLD"> <idno type="RNSR">200817410U</idno> <orgName>Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques</orgName> <orgName type="acronym">IP-TPT</orgName> <date type="start">2008-01-01</date> <date type="end">2017-12-31</date> <desc> <address> <addrLine>Faculté de Pharmacie27 bd Jean Moulin CS 3006413385 Marseille </addrLine> <country key="FR"></country> </address> <ref type="url">http://pharmacie.univ-amu.fr/umr-md3</ref> </desc> <listRelation> <relation name="UMR MD3" active="#struct-300092" type="direct"></relation> <relation name="UMR MD3" active="#struct-300073" type="direct"></relation> <relation name="UMR MD3" active="#struct-198056" type="direct"></relation> <relation name="UMR MD3" active="#struct-67872" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR MD3" active="#struct-300092" type="direct"><org type="institution" xml:id="struct-300092" status="VALID"> <orgName>Service de Santé des Armées</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR MD3" active="#struct-300073" type="direct"><org type="institution" xml:id="struct-300073" status="VALID"> <orgName>Assistance Publique - Hôpitaux de Marseille</orgName> <orgName type="acronym">APHM</orgName> <desc> <address> <addrLine>Direction générale AP-HM80, rue Brochier13 354 Marseille Cedex 5</addrLine> <country key="FR"></country> </address> <ref type="url">http://fr.ap-hm.fr/</ref> </desc> </org></tutelle><tutelle name="UMR MD3" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno> <idno type="ISNI">0000 0001 2176 4817</idno> <orgName>Aix Marseille Université</orgName> <orgName type="acronym">AMU</orgName> <date type="start">2012-01-01</date> <desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-amu.fr/</ref> </desc> </org></tutelle><tutelle name="UMR MD3" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName> <orgName type="acronym">IRD</orgName> <desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.ird.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Larhsini, Mustapha" sort="Larhsini, Mustapha" uniqKey="Larhsini M" first="Mustapha" last="Larhsini">Mustapha Larhsini</name></author><author><name sortKey="Markouk, Mohamed" sort="Markouk, Mohamed" uniqKey="Markouk M" first="Mohamed" last="Markouk">Mohamed Markouk</name></author><author><name sortKey="Ollivier, Evelyne" sort="Ollivier, Evelyne" uniqKey="Ollivier E" first="Evelyne" last="Ollivier">Evelyne Ollivier</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-532732" status="INCOMING"> <orgName>Laboratory of Pharmacognosy and Ethnopharmacology</orgName> <desc> <address> <addrLine>UMR-MD3, Faculty of Pharmacy, 27, bd Jean-Moulin, CS 30064, 13385, Marseille cedex 5</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Bekkouche, Khalid" sort="Bekkouche, Khalid" uniqKey="Bekkouche K" first="Khalid" last="Bekkouche">Khalid Bekkouche</name></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:hal-01765799</idno><idno type="halId">hal-01765799</idno><idno type="halUri">https://hal-amu.archives-ouvertes.fr/hal-01765799</idno><idno type="url">https://hal-amu.archives-ouvertes.fr/hal-01765799</idno><idno type="doi">10.1016/j.eujim.2017.08.009</idno><date when="2017-09">2017-09</date><idno type="wicri:Area/Hal/Corpus">000135</idno><idno type="wicri:Area/Hal/Curation">000135</idno><idno type="wicri:Area/Hal/Checkpoint">000048</idno><idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000048</idno><idno type="wicri:doubleKey">18763820:2017:El Bouzidi L:in:vitro:antiplasmodial</idno><idno type="wicri:Area/Main/Merge">000C63</idno><idno type="wicri:Area/Main/Curation">000C63</idno><idno type="wicri:Area/Main/Exploration">000C63</idno><idno type="wicri:Area/France/Extraction">000092</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">In vitro antiplasmodial activity of Withania frutescens —Solanaceae</title><author><name sortKey="El Bouzidi, Laila" sort="El Bouzidi, Laila" uniqKey="El Bouzidi L" first="Laila" last="El Bouzidi">Laila El Bouzidi</name></author><author><name sortKey="Ben Bakrim, Widad" sort="Ben Bakrim, Widad" uniqKey="Ben Bakrim W" first="Widad" last="Ben Bakrim">Widad Ben Bakrim</name></author><author><name sortKey="Mahiou Leddet, Valerie" sort="Mahiou Leddet, Valerie" uniqKey="Mahiou Leddet V" first="Valerie" last="Mahiou-Leddet">Valerie Mahiou-Leddet</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-188653" status="VALID"> <idno type="RNSR">201220339K</idno> <orgName>Institut méditerranéen de biodiversité et d'écologie marine et continentale</orgName> <orgName type="acronym">IMBE</orgName> <date type="start">2012</date> <desc> <address> <addrLine>Aix Marseille Université, Campus Etoile, Faculté St-Jérôme case 421 Av. . escadrille Normandie-Niemen 13397 MARSEILLE CEDEX 20</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.imbe.fr/</ref> </desc> <listRelation> <relation name="UMR7263" active="#struct-195507" type="direct"></relation> <relation name="UMR7263" active="#struct-198056" type="direct"></relation> <relation name="UMR7263" active="#struct-302715" type="direct"></relation> <relation name="UMR7263" active="#struct-441569" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR7263" active="#struct-195507" type="direct"><org type="institution" xml:id="struct-195507" status="VALID"> <orgName>Avignon Université</orgName> <orgName type="acronym">AU</orgName> <desc> <address> <addrLine>74 rue Louis Pasteur - 84 029 Avignon cedex 1</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-avignon.fr/</ref> </desc> </org></tutelle><tutelle name="UMR7263" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno> <idno type="ISNI">0000 0001 2176 4817</idno> <orgName>Aix Marseille Université</orgName> <orgName type="acronym">AMU</orgName> <date type="start">2012-01-01</date> <desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-amu.fr/</ref> </desc> </org></tutelle><tutelle name="UMR7263" active="#struct-302715" type="direct"><org type="institution" xml:id="struct-302715" status="VALID"> <orgName>Institut de recherche pour le développement [IRD] : UMR237</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR7263" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Azas, Nadine" sort="Azas, Nadine" uniqKey="Azas N" first="Nadine" last="Azas">Nadine Azas</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-220808" status="OLD"> <idno type="RNSR">200817410U</idno> <orgName>Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques</orgName> <orgName type="acronym">IP-TPT</orgName> <date type="start">2008-01-01</date> <date type="end">2017-12-31</date> <desc> <address> <addrLine>Faculté de Pharmacie27 bd Jean Moulin CS 3006413385 Marseille </addrLine> <country key="FR"></country> </address> <ref type="url">http://pharmacie.univ-amu.fr/umr-md3</ref> </desc> <listRelation> <relation name="UMR MD3" active="#struct-300092" type="direct"></relation> <relation name="UMR MD3" active="#struct-300073" type="direct"></relation> <relation name="UMR MD3" active="#struct-198056" type="direct"></relation> <relation name="UMR MD3" active="#struct-67872" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR MD3" active="#struct-300092" type="direct"><org type="institution" xml:id="struct-300092" status="VALID"> <orgName>Service de Santé des Armées</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="UMR MD3" active="#struct-300073" type="direct"><org type="institution" xml:id="struct-300073" status="VALID"> <orgName>Assistance Publique - Hôpitaux de Marseille</orgName> <orgName type="acronym">APHM</orgName> <desc> <address> <addrLine>Direction générale AP-HM80, rue Brochier13 354 Marseille Cedex 5</addrLine> <country key="FR"></country> </address> <ref type="url">http://fr.ap-hm.fr/</ref> </desc> </org></tutelle><tutelle name="UMR MD3" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno> <idno type="ISNI">0000 0001 2176 4817</idno> <orgName>Aix Marseille Université</orgName> <orgName type="acronym">AMU</orgName> <date type="start">2012-01-01</date> <desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-amu.fr/</ref> </desc> </org></tutelle><tutelle name="UMR MD3" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID"> <orgName>Institut de Recherche pour le Développement</orgName> <orgName type="acronym">IRD</orgName> <desc> <address> <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.ird.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Larhsini, Mustapha" sort="Larhsini, Mustapha" uniqKey="Larhsini M" first="Mustapha" last="Larhsini">Mustapha Larhsini</name></author><author><name sortKey="Markouk, Mohamed" sort="Markouk, Mohamed" uniqKey="Markouk M" first="Mohamed" last="Markouk">Mohamed Markouk</name></author><author><name sortKey="Ollivier, Evelyne" sort="Ollivier, Evelyne" uniqKey="Ollivier E" first="Evelyne" last="Ollivier">Evelyne Ollivier</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-532732" status="INCOMING"> <orgName>Laboratory of Pharmacognosy and Ethnopharmacology</orgName> <desc> <address> <addrLine>UMR-MD3, Faculty of Pharmacy, 27, bd Jean-Moulin, CS 30064, 13385, Marseille cedex 5</addrLine> <country key="FR"></country> </address> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Bekkouche, Khalid" sort="Bekkouche, Khalid" uniqKey="Bekkouche K" first="Khalid" last="Bekkouche">Khalid Bekkouche</name></author></analytic><idno type="DOI">10.1016/j.eujim.2017.08.009</idno><series><title level="j">European Journal of Integrative Medicine</title><idno type="ISSN">18763820</idno><imprint><date type="datePub">2017-09</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Antiplasmodial activity</term><term>Cytotoxicity</term><term>Plasmodium falciparum</term><term>Selectivity index</term><term>Withania frutescens</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>Introduction: Drugs resistant Plasmodium falciparum is a recurring issue that threatens public health. New anti-plasmodial drugs are needed to overcome this problem. The aim of this study was to characterize in vitro an-tiplasmodial activity of Withania frutescens (Solanaceae) against chloroquine-resistant Plasmodium falciparum strain. Methods: The in vitro antiplasmodial activity of leaves and roots extracts from Withania frutescens was performed in 96 well plates and preliminary phytochemical analysis was performed for the active fractions. The toxicity of the plant extract against CHO (Chinese Hamster Ovary) cells was assessed using the tetrazolium salt MTT col-orimetric assay. The selectivity index (SI) was calculated as the ratio between the cytotoxic and antiparasitic activities. Results: The methanol extract of W. frutescens leaves showed a good antiplasmodial activity (CC 50 18.1 μg/ml). Furthermore, ethyl acetate and butanol fractions showed promising in vitro antiplasmodial activity with a se-lectivity index of 3.1 and 2.4, respectively. The roots of W. frutescens were found to be inactive with a CC 50 value > 80 μg/ml. Conclusion: The antiplasmodial activity of W. frutescens may in part be attributed to the presence of polyphenolic and flavonoid compounds. Based on our results, ethyl acetate and butanol leaves fractions could be considered as a promising source for the development of putative antiplasmodial drugs.</p> </div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement><orgName><li>Université d'Aix-Marseille</li></orgName></list><tree><noCountry><name sortKey="Bekkouche, Khalid" sort="Bekkouche, Khalid" uniqKey="Bekkouche K" first="Khalid" last="Bekkouche">Khalid Bekkouche</name><name sortKey="Ben Bakrim, Widad" sort="Ben Bakrim, Widad" uniqKey="Ben Bakrim W" first="Widad" last="Ben Bakrim">Widad Ben Bakrim</name><name sortKey="El Bouzidi, Laila" sort="El Bouzidi, Laila" uniqKey="El Bouzidi L" first="Laila" last="El Bouzidi">Laila El Bouzidi</name><name sortKey="Larhsini, Mustapha" sort="Larhsini, Mustapha" uniqKey="Larhsini M" first="Mustapha" last="Larhsini">Mustapha Larhsini</name><name sortKey="Markouk, Mohamed" sort="Markouk, Mohamed" uniqKey="Markouk M" first="Mohamed" last="Markouk">Mohamed Markouk</name></noCountry><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Mahiou Leddet, Valerie" sort="Mahiou Leddet, Valerie" uniqKey="Mahiou Leddet V" first="Valerie" last="Mahiou-Leddet">Valerie Mahiou-Leddet</name></region><name sortKey="Azas, Nadine" sort="Azas, Nadine" uniqKey="Azas N" first="Nadine" last="Azas">Nadine Azas</name><name sortKey="Ollivier, Evelyne" sort="Ollivier, Evelyne" uniqKey="Ollivier E" first="Evelyne" last="Ollivier">Evelyne Ollivier</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000092 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000092 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= France
|étape= Analysis
|type= RBID
|clé= Hal:hal-01765799
|texte= In vitro antiplasmodial activity of Withania frutescens —Solanaceae
}}
| This area was generated with Dilib version V0.6.33. |  |